Brucellosis is an important zoonotic disease caused by the facultative intracellular Gram-negative Brucella species. The genus Brucella belongs to the family Brucellaceae under order Rhizobiales of Alphaproteobacteria. Brucella was named after David Bruce, who first isolated the bacterium from the spleen of a fatal case of Brucellosis in Malta in 1886, which was then known as Malta fever. Brucella infects a broad range of mammals, ranging from dolphins, domestic animals to humans. Brucellosis is an endemic disease having high morbidity and significant economic impact in many developing countries such as Africa, Asia, and Latin America.
Brucellosis is transmitted to humans from infected animals through direct contact and also by consumption of unpasteurized dairy products from diseased animals. In human, this disease is characterized by an acute phase with undulant fever and chronic phase by intracellular localization of the pathogen that causes severe damage to organs. Clinical complications of Brucellosis include neurological disorders, oscillating fever, endocarditis and osteoarticular complications. Infected patients require hospitalization and remain ill for weeks or even months.
Ten different Brucella species are recognized based on the pathogenicity and host preference, which include B. melitensis (goats and sheep), B. abortus (cattle and bison), B. suis (infecting primarily swine but also hares, rodents, and reindeer), B. ovis (sheep), B. canis (dogs), B. neotomae (wood rats), B. ceti (cetaceans) and B. pinnipedialis (pinnipeds), B. microti (common vole) and B. inopinata (Human). Brucella genome has two circular chromosomes of ~2.1 MB and ~1.2 MB in size and has a GC content of 57.2%. Brucella genome has ~3,200 - 3,400 ORFs.
BrucellaBase is providing features of a genome database together with unique analysis tools. We have developed a web version of the multilocus sequence typing (MLST) (Whatmore et al., 2007) and phylogenetic analysis of Brucella spp. BrucellaBase currently contains genome data of 510 Brucella strains along with the user interfaces for BLAST, VFDB, ARDB, pairwise genome alignment and MLST typing. Availability of these tools will enable the veterinary biologists to get meaningful information from Brucella genome sequences. BrucellaBase will regularly be updated with new genome sequences, new features along with improvements in genome annotations. Availability of these tools will enable the veterinary biologists to get meaningful information from Brucella genomes. BrucellaBase will regularly be updated with new genome sequences, new features along with improvements in genome annotations.